期刊
FEBS LETTERS
卷 583, 期 4, 页码 797-800出版社
WILEY
DOI: 10.1016/j.febslet.2009.01.041
关键词
Expressed sequence tag; Mate pair; Assembly; Database
资金
- National Institute of Agrobiological Sciences
- Ministry of Agriculture, Forestry and Fisheries of Japan
Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structures as well as protein-coding regions. As a result, we predicted 4480 protein-coding sequences, which were more accurate than ab initio predictions. Moreover, cross-species comparisons suggested that our predicted proteins were nearly complete. The cDNA clones obtained in this study will be important for further experimental studies. Our genome annotation is available at http://www.mg.dna.affrc.go.jp/. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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