期刊
FEBS LETTERS
卷 583, 期 4, 页码 670-674出版社
WILEY
DOI: 10.1016/j.febslet.2009.01.016
关键词
Cryptophyte (Rhodomonas salina); Fluorescence recovery after photobleaching; Protein diffusion; Thylakoid lumen; Phycobilin; Phycoerythrin
资金
- GAAV [A608170603]
- GACR [206/09/P094]
- Biotechnology and Biological Sciences Research Council
- Wellcome Trust
- [AV0Z50200510]
- [MSM6007665808]
- Biotechnology and Biological Sciences Research Council [C17403/2] Funding Source: researchfish
The thylakoid lumen is an important photosynthetic compartment which is the site of key steps in photosynthetic electron transport. The fluidity of the lumen could be a major constraint on photosynthetic electron transport rates. We used Fluorescence Recovery After Photobleaching in cells of the cryptophyte alga Rhodomonas salina to probe the diffusion of phycoerythrin in the lumen and chlorophyll complexes in the thylakoid membrane. In neither case was there any detectable diffusion over a timescale of several minutes. This indicates very restricted phycoerythrin mobility. This may be a general feature of protein diffusion in the thylakoid lumen. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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