期刊
FEBS LETTERS
卷 583, 期 9, 页码 1399-1402出版社
WILEY
DOI: 10.1016/j.febslet.2009.03.067
关键词
Hydrogen peroxide; Reactive oxygen species; Peroxiredoxin; Thioredoxin; Thioredoxin reductase
资金
- Ministerio de Educacion y Ciencia [BIO2007-60644]
- Junta de Andalucia (Spain) [P06-CVI-01578, BIO-182]
- Ministerio de Educacion y Ciencia
NADPH thioredoxin reductase C ( NTRC) is an interesting NTR with a thioredoxin (Trx) domain at the C-terminus, able to conjugate both activities for 2-Cys peroxiredoxin (Prx) reduction. NTRC is dimeric in the presence of NADPH and interacted with dimeric 2-Cys Prx through the Trx module by a mixed disulfide between Cys377 of NTRC and Cys61 of the 2-Cys Prx. NTRC variants of both NTR and Trx active sites were inactive, but 1: 1 mixtures of both variants allowed partial recovery of activity suggesting inter-subunit transfer of electrons during catalysis. Based on these results we propose a model for the reaction mechanism of NTRC.
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