期刊
FEBS LETTERS
卷 584, 期 1, 页码 119-123出版社
WILEY
DOI: 10.1016/j.febslet.2009.11.014
关键词
Glucosinolate; Sulfate assimilation; Adenosine phosphosulfate kinase; Reverse genetic
资金
- British Biotechnology and Biological Sciences Research Council [BB/D009596/1]
- BBSRC [BB/D009596/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D009596/1] Funding Source: researchfish
In Arabidopsis thaliana, adenosine-5'-phosphosulfate kinase (APK) provides activated sulfate for sulfation of secondary metabolites, including the glucosinolates. We have successfully isolated three of the four possible triple homozygous mutant combinations of this family. The APK1 isoform alone was sufficient to maintain WT levels of growth and development. Analysis of apk1 apk2 apk3 and apk1 apk3 apk4 mutants suggests that APK3 and APK4 are functionally redundant, despite being located in cytosol and plastids, respectively. We were, however, unable to isolate apk1 apk3 apk4 mutants, most probably because the apk1 apk3 apk4 triple mutant combination is pollen lethal. Therefore, we conclude that APS kinase is essential for plant reproduction and viability. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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