期刊
FEBS LETTERS
卷 582, 期 7, 页码 1087-1092出版社
WILEY
DOI: 10.1016/j.febslet.2008.02.062
关键词
malaria; resonance Raman spectroscopy; chloroquine; chemometrics; haemozoin
Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B-1g modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ are observed. The intensity changes are attributed to intermolecular drug binding of the CQ in a sandwich type complex between ferriprotoporphyrin IX (FePPIX) dimer units. It is postulated that the CQ binds via pi-pi interactions between adjacent and orientated porphyrins thereby disrupting the haemozoin aggregate and reducing excitonic interactions between adjacent haems. The results show the potential of Raman microscopy as a screening tool for FePPIX:drug interactions in live cells. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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