期刊
FEBS LETTERS
卷 582, 期 16, 页码 2382-2386出版社
WILEY
DOI: 10.1016/j.febslet.2008.05.046
关键词
neprilysin; neprilysin-2; M13 metallopeptidase; thiorphan; phosphoramidon
资金
- MRC [G9824728] Funding Source: UKRI
- Medical Research Council [G9824728] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
- Medical Research Council [G9824728] Funding Source: Medline
Neprilysin-2 (NEP2) is a novel metallopeptidase homologous to neprilysin (NEP), an enzyme involved in regulation of neuropeptide signalling. NEP2 exists as two alternatively spliced isoforms, NEP2 and NEP2 D. In this study, we cloned and expressed both human isoforms. Human NEP2 exists as a membrane-bound and soluble enzyme, whereas human NEP2 D exists as two membrane-bound glycoforms, localised to the ER and plasma membrane. Surprisingly, NEP2 substrate specificity and inhibitor binding was distinct from that of human NEP, suggesting that NEP and NEP2 play distinct physiological roles in humans, and human NEP2 differs markedly from its rodent homologues. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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