期刊
FEBS LETTERS
卷 582, 期 12, 页码 1743-1748出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2008.04.042
关键词
diacylglycerol kinases; integral protein; hydrophobic domain; GFP; membrane sequestering; Arabidopsis thaliana
Diacylglycerol kinases (DGKs) catalyze the phosphorylation of diacylglycerol into phosphatidic acid. To fulfill their role in many signalling processes, DGKs must be located at, or in, membranes. Most mammalian DGKs are cytosolic and are recruited to membranes upon stimulation, except for epsilon type DGKs that are permanently membrane-associated through a hydrophobic segment. Nothing is known about the mechanism( s) involved in the membrane localization of plant DGKs. By fusion to fluorescent proteins, we show that two DGKs from cluster I in Arabidopsis thaliana possess amino-terminal hydrophobic segments that are sufficient to address them to endoplasmic reticulum membranes. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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