期刊
FEBS LETTERS
卷 582, 期 5, 页码 826-828出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2008.02.007
关键词
apolipoprotein M; signal peptide; type I signal peptidase; gel chromatography; high density lipoprotein
Apolipoprotein M (apoM) is predominantly associated with HDL. In this study, it was investigated whether apoM's uncleaved signal peptide is necessary for the protein's ability to associate with lipoproteins. ApoM with a cleavable signal peptide, Q22A, was expressed, together with wild-type apoM, in HEK293 cells. On size-exclusion chromatography, the elution profile of wild-type apoM was similar to that of human HDL-associated plasma apoM. In contrast, the size of the Q22A mutant corresponded to free, unassociated apoM. This strongly indicates that the signal peptide is indeed necessary for apoM's ability to associate with lipid. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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