期刊
FEBS LETTERS
卷 582, 期 29, 页码 4100-4106出版社
WILEY
DOI: 10.1016/j.febslet.2008.11.006
关键词
Haloperoxidase; Oxygenation; P450; Pyridine; Pyridine N-oxide; Agrocybe
资金
- German Ministry of Research and Education (BMBF)
- German Environmental Foundation (DBU)
Agrocybe aegerita peroxidase (AaP) is a versatile extracellular biocatalyst that can oxygenate aromatic compounds. Here, we report on the selective oxidation of pyridine (PY) yielding pyridine N-oxide as sole product. Using (H2O2)-O-18 as co-substrate, the origin of oxygen was confirmed to be the peroxide. Therefore, AaP can be regarded as a true peroxygenase transferring one oxygen atom from peroxide to the substrate. To our best knowledge, there are only two types of enzymes oxidizing PY at the nitrogen: bacterial methane monooxygenase and a few P450 monooxygenases. AaP is the first extracellular enzyme and the first peroxidase that catalyzes this reaction, and it converted also substituted PYs into the corresponding N-oxides. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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