期刊
FEBS LETTERS
卷 582, 期 21-22, 页码 3243-3248出版社
WILEY
DOI: 10.1016/j.febslet.2008.08.023
关键词
osteoclastogenesis; nuclear factor of activated T; cells c1; RAW 264.7 cell line; receptor activator of nuclear factor-kappa B ligand; integrin
资金
- Japan Society for the Promotion of Science, Tokyo, Japan [20390470, 20659306, 20390463]
- Grants-in-Aid for Scientific Research [20390463, 20390470, 20659306] Funding Source: KAKEN
Multinuclear osteoclasts are derived from CD11b-positive mononuclear cells in bone marrow and in circulation. FACS sorting experiments showed impaired osteoclastogenesis in RAW264.7 cells with low CD11b expression. Neutralizing antibodies and siRNA against CD11b inhibited osteoclastogenesis induced by RANKL. Although primary cultured mouse bone marrow macrophages expressed CD11a and CD11b, osteoclastogenesis induced by M-CSF and RANKL was inhibited in the presence of anti-CD11b or anti-CD18 but not anti-CD11a antibodies. Furthermore, anti-CD11b antibodies inhibited NFATc1 expression induced by M-CSF and RANKL in BMMs. These. findings suggest, at least partly, an important role of CD11b in osteoclastogenesis. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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