期刊
FEBS JOURNAL
卷 286, 期 4, 页码 642-652出版社
WILEY
DOI: 10.1111/febs.14666
关键词
gene regulation; microRNA target; microRNA target validation; miRISC
资金
- Natural Sciences and Engineering Research Council
- Canadian Institutes of Health Research
As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.
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