期刊
FEBS JOURNAL
卷 280, 期 13, 页码 3028-3049出版社
WILEY
DOI: 10.1111/febs.12181
关键词
CBM33; cellulase; cellulose; chitin; chitinase; chitobiase; chitosan; GH61; lytic polysaccharide monooxygenase; processivity
资金
- Research Council of Norway [186946, 196885, 209335, 177542, 214138]
The chitinolytic machinery of Serratiamarcescens is one of the best known enzyme systems for the conversion of insoluble polysaccharides. This machinery includes four chitin-active enzymes: ChiC, an endo-acting non-processive chitinase; ChiA and ChiB, two processive chitinases moving along chitin chains in opposite directions; and CBP21, a surface-active CBM33-type lytic polysaccharide monooxygenase that introduces chain breaks by oxidative cleavage. Furthermore, an N-acetylhexosaminidase or chitobiase converts the oligomeric products from the other enzymes to monomeric N-acetylglucosamine. Here we discuss the catalytic mechanisms of these enzymes as well as the structural basis of each enzyme's specific role in the chitin degradation process. We also discuss how knowledge of this enzyme system may be extrapolated to other enzyme systems for conversion of insoluble polysaccharides, in particular conversion of cellulose by cellulases and GH61-type lytic polysaccharide monooxygenases.
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