期刊
FEBS JOURNAL
卷 279, 期 17, 页码 3185-3191出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1742-4658.2012.08698.x
关键词
Bacteroides thetaiotaomicron VPI-5482; cycloisomaltooligosaccharide glucanotransferase; endo-dextranase; glycoside hydrolase family 66; isomaltotetraose
资金
- Japan Society for the Promotion of Science
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)
- Grants-in-Aid for Scientific Research [21405020, 24658089] Funding Source: KAKEN
Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.
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