期刊
FEBS JOURNAL
卷 278, 期 10, 页码 1745-1756出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1742-4658.2011.08099.x
关键词
Escherichia coli; RNase III; rRNA mutation; rRNA processing; translation
资金
- SIDA [SWE-2006-509]
- Swedish Institute [01146/2006]
- Carl Trygger Foundation [CTS 08:159]
Genetic selection has been used to isolate second-site suppressors of a defective cold-sensitive initiation factor I (IF1) R69L mutant of Escherichia coli. The suppressor mutants specifically map to a single rRNA operon on a plasmid in a strain with all chromosomal rRNA operons deleted. Here, we describe a set of suppressor mutations that are located in the processing stem of precursor 23S rRNA. These mutations interfere with processing of the 23S rRNA termini. A lesion of RNase III also suppresses the cold sensitivity. Our results suggest that the mutant IF1 strain is perturbed at the level of ribosomal subunit association, and the suppressor mutations partially compensate for this defect by disrupting rRNA maturation. These results support the notion that IF1 is an RNA chaperone and that translation initiation is coupled to ribosomal maturation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据