期刊
FASEB JOURNAL
卷 28, 期 6, 页码 2645-2654出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.13-247791
关键词
innate immunity; alginate; phagocytosis; NF-kappa B; nitric oxide
资金
- National Natural Science Foundation of China [31000770]
- Foundation for Distinguished Young Talents in Higher Education (Guangdong, China
- FDYT) [LYM10114]
- Marine Biotechnology Initiative Project (Shenzhen, China)
- Grants-in-Aid for Scientific Research [12F02093] Funding Source: KAKEN
Alginate from marine seaweeds is receiving continuous attention owing to its wide physiological activities. Herein, we sought to elucidate possible effects of alginate-derived polyguluronate (PG) and unsaturated guluronate oligosaccharide (GOS) on antibacterial activities of macrophages. Our results showed that, in contrast to PG, GOS markedly increased the phagocytosis of IgG-opsonized Escherichia coli and Staphylococcus aureus and further inhibited the survival of intracellular bacteria in macrophages. In line with this, GOS treatment resulted in the enhanced expression of Fc gamma receptors on macrophages. In addition, GOS activated NF-kappa B pathway, induced TNF-alpha secretion, and elevated the expression of inducible nitric oxide synthase and the production of nitric oxide. Meanwhile, GOS stimulated the production of reactive oxygen species in macrophages. Moreover, guluronate trimer to hexamer (G3-G6) in GOS exhibited significant activity that increased the bacterial phagocytosis of macrophages, with the pentamer (G5), displaying the highest activity. Finally, our in vivo results further confirmed that GOS but not PG significantly improved bacterial clearance in murine acute peritonitis. In conclusion, GOS enhances antibacterial activities of macrophages via modulating signaling pathways related to innate immunity, suggesting that GOS might be a promising therapeutic candidate to improve the host defense against bacterial infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据