期刊
FASEB JOURNAL
卷 27, 期 9, 页码 3478-3486出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.12-217984
关键词
lipid rafts; OGT; PI3K pathway
资金
- University of Lille 1
- Centre National de la Recherche Scientifique (CNRS)
- Franco-Mexican exchange program ECOS NORD [M09S02]
- Rosalind Franklin fellowship (Instituto de Ciencia y Tecnologia del Distrito Federal)
Lipid microdomains (rafts) are cholesterol-enriched dynamic ordered lipid domains belonging to cell membranes involved in diverse cellular functions, including signal transduction, membrane trafficking, and infection. Many studies have reported relationships between insulin signaling and lipid rafts. Likewise, links between insulin signaling and O-GlcNAcylation have also been described. However, the potential connection between O-GlcNAc and raft dynamics remains unexplored. Here we show that O-GlcNAc and the enzyme that creates this modification, O-GlcNAc transferase (OGT), are localized in rafts. On insulin stimulation, we observe time-dependent increases in OGT expression and localization within rafts. We show that these processes depend on activation of the phosphatidylinositol 3-kinase (PI3K) pathway. Inhibition of OGT does not significantly affect cholesterol synthesis and raft building but decreases insulin receptor expression and PI3K and mitogen-activated protein kinase pathway activation. Taken together, these findings indicate that O-GlcNAcylation, lipid rafts, and signaling pathways are spatiotemporally coordinated to enable fundamental cellular functions.Perez-Cervera, Y., Dehennaut, V., Aquino Gil, M., Guedri, K., Solorzano Mata, C. J., Olivier-Van Stichelen, S., Michalski, J.-C., Foulquier, F., Lefebvre, T. Insulin signaling controls the expression of O-GlcNAc transferase and its interaction with lipid microdomains.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据