期刊
FASEB JOURNAL
卷 25, 期 11, 页码 3815-3827出版社
WILEY
DOI: 10.1096/fj.11-187484
关键词
cell cycle progression; signal transduction; cell signaling
资金
- Royal Physiographic Society (Lund, Sweden)
- Ake Wiberg Foundations
- Thelma Zoegas Foundation
- Per-Eric Foundation
- Ulla Schyberg Foundation
- O. E. and Edla Johansson Foundation
- Gradfordska, H. and G. Jeassons Foundation
- O. and E. Ericsson Foundation
- Gyllenstiernska Krapperupsstiftelsen
- Universitetssjukhuset Malmo Allmana Sjukhus (U-MAS)
- U-MAS Cancer Research Fund
- Swedish Research Council
- Swedish Society for Medical Research
We show that the centrosome- and microtubule-regulating protein gamma-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. gamma-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. gamma-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-gamma-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of gamma-tubulin or by complex formation between gamma-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the gamma-tubulin C terminus encodes a DNA-binding domain that interacts with E2F-regulated promoters, resulting in gamma-tubulin-mediated transient activation of E2Fs. Thus, we report a novel mechanism regulating the activity of E2Fs, which can help explain how these proteins affect cell cycle progression in mammalian cells.-Hoog, G., Zarrizi, R., von Stedingk, K., Jonsson, K., Alvarado-Kristensson, M. Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. FASEB J. 25, 3815-3827 (2011). www.fasebj.org
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