4.7 Article

Thyrotropin powers human mitochondria

期刊

FASEB JOURNAL
卷 24, 期 5, 页码 1525-1531

出版社

WILEY
DOI: 10.1096/fj.09-147728

关键词

aging; hypothalamic-pituitary-thyroid axis; mitochondrial energy metabolism; oxygen utilization; skin

资金

  1. Manchester National Institute for Health Research (NIHR) Biomedical Research Center

向作者/读者索取更多资源

Here we demonstrate that the neuropeptide hormone thyrotropin (TSH), which controls thyroid hormone production, exerts a major nonclassical function in mitochondrial biology. Based on transcriptional, ultrastructural, immunohistochemical, and biochemical evidence, TSH up-regulates mitochondrial biogenesis and consequently activity in organ-cultured normal human epidermis in situ. Mitochondrial activity was assessed by measuring 2 key components of the respiratory chain. The abundance of mitochondria was assessed employing 2 independent morphological techniques: counting their numbers in human epidermis by high-magnification light microscopy of skin sections immunostained for mitochondria-selective cytochromec-oxidase subunit 1 (MTCO1) and transmission electron microscopy (TEM). Treatment with 10 mU/ml of TSH for 6 d strongly up-regulates the number of light-microscopically visualized, MTCO1-demarcated mitochondria. On the ultrastructural level, TEM confirms that TSH indeed stimulates mitochondrial proliferation and biogenesis in the perinuclear region of human skin epidermal keratinocytes. On the transcriptional level, TSH up-regulates MTCO1 mRNA (quantitative RT-PCR) and significantly enhances complex I and IV (cytochrome-c-oxidase) activity. This study pioneers the concept that mitochondrial energy metabolism and biogenesis in a normal, prototypic human epithelial tissue underlies potent neuroendocrine controls and introduces human skin organ culture as a clinically relevant tool for further exploring this novel research frontier in the control of mitochondrial biology.-Poeggeler, B., Knuever, J., Gaspar, E., Biro, T., Klinger, M., Bodo, E., Wiesner, R. J., Wenzel, B. E., Paus, R. Thyrotropin powers human mitochondria. FASEB J. 24, 1525-1531 (2010). www.fasebj.org

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据