期刊
FASEB JOURNAL
卷 24, 期 3, 页码 723-730出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.09-139790
关键词
ventral tegmental area; VTA; eGFP; stereotactic injection; cell-type specific reexpression; Cre/loxP recombination system
资金
- Agence Nationale pour la Recherche
- Institut Pasteur
- College de France
- Centre National de la Recherche Scientifique CNRS [URA 2182]
- Association de Recherche sur le Cancer (ARC)
- Institut National du Cancer (INCa)
- Mission Interministerielle de Lutte contre la Drogue et la Toxicomanie (MILDT)
- Fondation pour la Recherche Medicale (FRM)
- European Commission
- ANR-Neuro-GRADA
- EU-STREP-PheCOMP
- MILDT
- Region of Sardinia
- Fondation pour la Recherche Medicale FRM
Lentiviral expression vectors are powerful tools for gene therapy and long-term gene expression/repression in the mammalian brain. However, no specificity of transduction has been reported so far in the central nervous system. Here we have developed a novel system to achieve a neuronal subtype specific expression in either dopaminergic (DA) or GABAergic neurons. We employed a delivery strategy by which the transgene is not expressed until its activation by Cre recombinase. We successfully tested the system in vitro and then used this novel lentivector, containing loxP sites, in 2 different transgenic mouse lines expressing Cre either in DA or in GABAergic neurons. In both lines the reporter gene was detected exclusively in Cre-positive cells, demonstrating that with this experimental approach we were able to achieve completely specific expression of transgenes delivered by lentiviral vectors. This universal system can be applied to all neural subtypes making use of the growing number of specific Cre driver lines.-Tolu, S., Avale, M. E., Nakatani, H., Pons, S., Parnaudeau, S., Tronche, F., Vogt, A., Monyer, H., Vogel, R., de Chaumont, F., Olivo-Marin, J.-C., Changeux, J.-P., Maskos, U. A versatile system for the neuronal subtype specific expression of lentiviral vectors. FASEB J. 24, 723-730 (2010). www.fasebj.org
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