期刊
FASEB JOURNAL
卷 24, 期 1, 页码 309-317出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.09-137653
关键词
islet amyloid polypeptide; surface tension; hydrophobic-hydrophilic interfaces
资金
- Synaptica Ltd. (Didcot, Oxfordshire, UK)
- Glasstone Trust (Oxford, UK)
- MRC [G0801917] Funding Source: UKRI
- Medical Research Council [G0801917] Funding Source: researchfish
Amyloid accumulation is associated with pathological conditions, including type II diabetes and Alzheimer's disease. Lipids influence amyloidogenesis and are themselves targets for amyloid-mediated cell membrane disruption. Amyloid precursors are surface-active, accumulating at hydrophobic-hydrophilic interfaces (e. g., air-water), where their biophysical and kinetic behaviors differ from those in the bulk solution with significant and underappreciated consequences. Biophysical modeling predicted the probability and rate of beta-sheet amyloid dimer formation to be higher and faster at the air-water interface (AWI) than in the bulk (by 14 and similar to 1500 times, respectively). Time-course staining experiments with a typical amyloid dye verified our predictions by demonstrating that without AWI, islet amyloid polypeptide (IAPP) fibrilization was abolished or slowed, depending on the conditions. Our controls included undisturbed IAPP reactions, and we ascertained that the AWI removal process (technical or material) did not itself affect the reaction. Furthermore, we showed that the role of membranes in amyloidogenesis has been previously underestimated; in an in vivo-like situation (with no AWI), anionic liposomes (containing dioleoylphosphatidylglycerol) enhanced IAPP fibrilogenesis far more than described previously in conventional assay conditions (in the presence of an AWI). These findings have implications for the protein misfolding field and in assay design to target toxic protein aggregation.-Jean, L., Lee, C. F., Lee, C., Shaw, M., Vaux, D. J. Competing discrete interfacial effects are critical for amyloidogenesis. FASEB J. 24, 309-317 (2010). www.fasebj.org
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