期刊
FASEB JOURNAL
卷 23, 期 12, 页码 4276-4287出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.09-134965
关键词
MicroRNA; tumor; neuronal; retinoic acid
资金
- Italian Ministry of Instruction
- University and Scientific Research
- FIRB [RBNE01H3K5, RBNE01MBEC]
- Italy-USA Collaborative Programme
- Ministry of Health
- Associazione Italiana per la Ricerca sul Cancro
- National Research Council
- Ministry of University and Research
- Telethon [GGP07118]
MicroRNAs are a class of sophisticated regulators of gene expression, acting as post-transcriptional inhibitors that recognize their target mRNAs through base pairing with short regions along the 3'UTRs. Several microRNAs are tissue specific, suggesting a specialized role in tissue differentiation or maintenance, and quite a few are critically involved in tumorigenesis. We studied miR-128, a brain-enriched microRNA, in retinoic acid-differentiated neuroblastoma cells, and we found that this microRNA is up-regulated in treated cells, where it down-modulates the expression of two proteins involved in the migratory potential of neural cells: Reelin and DCX. Consistently, miR-128 ectopic overexpression suppressed Reelin and DCX, whereas the LNA antisense-mediated miR-128 knockdown caused the two proteins to increase. Ectopic miR-128 overexpression reduced neuroblastoma cell motility and invasiveness, and impaired cell growth. Finally, the analysis of a small series of primary human neuroblastomas showed an association between high levels of miR-128 expression and favorable features, such as favorable Shimada category or very young age at diagnosis. Thus, we provide evidence for a role for miR-128 in the molecular events modulating neuroblastoma progression and aggressiveness.-Evangelisti, C., Florian, M. C., Massimi, I., Dominici, C., Giannini, G., Galardi, S., Bue, M. C., Massalini, S., McDowell, H. P., Messi, E., Gulino, A., Farace, M. G., Ciafre, S. A. MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness. FASEB J. 23, 4276-4287 (2009). www.fasebj.org
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