Translocator protein (18 kDa) is involved in primitive erythropoiesis in zebrafish

The translocator protein (18 kDa) (TSPO), also known as peripheral-type benzodiazepine receptor, is directly or indirectly associated with many biological processes. Although extensively characterized, the specific function of TSPO during development remains unclear. It has been reported that TSPO is involved in a variety of mechanisms, including cell proliferation, apoptosis, regulation of mitochondrial functions, cholesterol transport and steroidogenesis, and porphyrin transport and heme synthesis. Although the literature has reported a murine knockout model, the experiment did not generate information because of early lethality. We then used the zebrafish model to address the function of tspo during development. Information about spatiotemporal expression showed that tspo has a maternal and a zygotic contribution which, during somatogenesis, seems to be erythroid restricted to the intermediate cell mass. Genetic and pharmacological approaches used to invalidate Tspo function resulted in embryos with specific erythropoietic cell depletion. Although unexpected, this lack of blood cells is independent of the Tspo cholesterol binding site and reveals a new in vivo key role for Tspo during erythropoiesis.-Rampon, C., Bouzaffour, M., Ostuni, M. A., Dufourcq, P., Girard, C., Freyssinet, J. M., Lacapere, J.-J., Schweizer-Groyer, G., Vriz, S. Translocator protein (18 kDa) is involved in primitive erythropoiesis in zebrafish. FASEB J. 23, 4181-4192 (2009). www.fasebj.org