期刊
FASEB JOURNAL
卷 22, 期 10, 页码 3696-3705出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.08-102590
关键词
angiogenesis; CD105; tumorigenesis; kidney tumor; progenitors; mesenchymal stem cells
资金
- Associazione Italian, per la Ricerca sin Cancro (AIRC)
- Italian Ministry of University and Research (MIUR) COFIN
- MIUR
- Italian Ministry of Health (Ricerca Finalizzata 02
- Progetto S. Paolo Oncologia
- Progetti Finalizzati Regione Piemonte, Oncoprot
The purpose of the present study was to search for the presence of a tumor-initiating stem cell population in renal carcinomas. Based on the recent identification of mesenchymal stem cells in normal kidneys, we sorted cells expressing the mesenchymal stem cell marker CD105 from 5 human renal carcinomas. Because the CD105(+) but not the CD105(-) population showed enhanced tumorigenicity when injected in severely compromised immunodeficient (SCID) mice, we cloned and characterized CD105(+) cells and evaluated their stemness, differentiative ability, and serial tumor generation. Characterization of the phenotype of CD105(+) clones revealed several stem cell properties: 1) clonogenic ability, 2) expression of nestin, Nanog, Oct4 stem cell markers, and lack of differentiative epithelial markers, 3) ability to grow in non-adhesive spheroids, 4) an vitro differentiation into epithelial and endothelial cell types, and 5) generation in vivo of serially transplantable carcinomas containing an undifferentiated CD105(+) tumorigenic and a differentiated CD105(-) nontumorigenic population. In addition, some vessels present in carcinomas generated from CD105(+) clones were of human origin, suggesting the capability of tumor-initiating stem cells to in vivo differentiate also in endothelial cells. In conclusion, we demonstrate that CD105(+) cells and clones derived from renal carcinomas were enriched in tumor-initiating cells with stem characteristics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据