期刊
FARADAY DISCUSSIONS
卷 161, 期 -, 页码 91-111出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2fd20119d
关键词
-
资金
- Engineering and Physical Sciences Research Council [EP/J017566/1] Funding Source: researchfish
- EPSRC [EP/J017566/1] Funding Source: UKRI
In this paper, we demonstrate that it is possible to observe many aspects of critical phenomena in supported lipid bilayers using atomic force microscopy (AFM) with the aid of stable and precise temperature control. The regions of criticality were determined by accurately measuring and calculating phase diagrams for the 2 phase L-d-L-o region, and tracking how it moves with temperature, then increasing the sampling density around the estimated critical regions. Compositional fluctuations were observed above the critical temperature (T-c) and characterised using a spatial correlation function. From this analysis, the phase transition was found to be most closely described by the 2D Ising model, showing it is a critical transition. Below T-c roughening of the domain boundaries occurred due to the reduction in line tension close to the critical point. Smaller scale density fluctuations were also detected just below T-c. At T-c, we believe we have observed fluctuations on length scales greater than 10 mu m. The region of critically fluctuating 10-100 nm nanodomains has been found to extend a considerable distance above T-c to temperatures within the biological range, and seem to be an ideal candidate for the actual structure of lipid rafts in cell membranes. Although evidence for this idea has recently emerged, this is the first direct evidence for nanoscale domains in the critical region.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据