期刊
FARADAY DISCUSSIONS
卷 139, 期 -, 页码 143-159出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b717431d
关键词
-
资金
- Department of Engineering Materials, University of Sheffield
- Engineering and Physical Sciences Research Council [EP/E03103X/1] Funding Source: researchfish
- EPSRC [EP/E03103X/1] Funding Source: UKRI
We have recently achieved efficient cytosolic delivery by using pH-sensitive poly(2-(methacryloyloxy) ethylphosphorylcholine)-co-poly(2-( diisopropylamino)ethylmethacrylate) (PMPC-PDPA) diblock copolymers that self-assemble to form vesicles, known as polymersomes, in aqueous solution. It is particularly noteworthy that these diblock copolymers form stable polymersomes at physiological pH but rapidly dissociate below pH 6 to give molecularly-dissolved copolymer chains (unimers). These PMPC-PDPA polymersomes are used to encapsulate nucleic acids for efficient intracellular delivery. Confocal laser scanning microscopy and fluorescence flow cytometry are used to quantify cellular uptake and to study the kinetics of this process. Finally, we examine how PMPC-PDPA polymersomes affect the viability of primary human cells (human dermal fibroblasts (HDF)), paying particular regard to whether inflammatory responses are triggered.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据