期刊
ACS NANO
卷 9, 期 4, 页码 4636-4648出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01179
关键词
graphene; 3D printing; tissue engineering; neurogenesis
类别
资金
- NCI CCSG [P30 CA060553]
- NSF [DMR-1121262, EEC-01180251003]
- Northwestern University Mouse Histology and Phenotyping Laboratory
- NCI [CA060553]
- U.S. Army Research Office
- U.S. Army Medical Research and Material Command
- Northwestern University
- Northwestern University's International Institute for Nanotechnology (NU) [SP0030341]
- Northwestern University's McCormick Research Catalyst Award
- Office of Naval Research MURI Program [N00014-11-1-0690]
- Department of Defense (DoD) through National Defense Science and Engineering Graduate (NDSEG) Fellowship Program
The exceptional properties of graphene enable applications in electronics, optoelectronics, energy storage, and structural composites. Here we demonstrate a 3D printable graphene (3DG) composite consisting of majority graphene and minority polylactide-co-glycolide, a biocompatible elastomer, 3D-printed from a liquid ink. This ink can be utilized under ambient conditions via extrusion-based 3D printing to create graphene structures with features as small as 100 mu m composed of as few as two layers (<300 mu m thick object) or many hundreds of layers (>10 cm thick object). The resulting 3DG material is mechanically robust and flexible while retaining electrical conductivities greater than 800 S/m, an order of magnitude increase over previously reported 3D-printed carbon materials. In vitro experiments in simple growth medium, in the absence of neurogenic stimuli, reveal that 3DG supports human mesenchymal stem cell (hMSC) adhesion, viability, proliferation, and neurogenic differentiation with significant upregulation of glial and neuronal genes. This coincides with hMSCs adopting highly elongated morphologies with features similar to axons and presynaptic terminals. in vivo experiments indicate that 3DG has promising biocompatibility over the course of at least 30 days. Surgical tests using a human cadaver nerve model also illustrate that 3D6 has exceptional handling characteristics and can be intraoperatively manipulated and applied to fine surgical procedures. With this unique set of properties, combined with ease of fabrication, 3DG could be applied electronic, biological, and bioelectronic medical and nonmedical devices.
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