期刊
EYE
卷 22, 期 11, 页码 1442-1445出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/eye.2008.176
关键词
human papillomavirus; pterygium; papilloma; PCR; hybrid capture assay; HC-II
Aim To elucidate the putative role of human papillomavirus (HPV) infection in pterygium and conjunctival papilloma. Methods Hybrid capture II (HC-II) and polymerase chain reaction (PCR) assays were performed to detect HPV in pterygium (42 samples obtained from 40 patients) and conjunctival papilloma (8 samples from 6 patients). The amount of HPV DNA was evaluated by measurement of relative light units (RLUs) on a luminometer. Results All papilloma samples were positive for HPV DNA by PCR and HC-II. The RLU values for specimens of recurrent and re-recurrent papilloma were markedly higher than those for specimens of primary lesions. HPV was detected by PCR in 2 of 42 (4.8%) beta-globin-positive pterygium specimens, whereas HC-II showed that HPV was negative in all pterygium samples. Conclusions Our results support the hypothesis that HPV DNA is associated with the pathogenesis of conjunctival papilloma, but not pterygium. RLU measurement by HC-II may serve as a marker for evaluating the activity of HPV in conjunctival tumours.
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