期刊
EXTREMOPHILES
卷 17, 期 3, 页码 453-461出版社
SPRINGER JAPAN KK
DOI: 10.1007/s00792-013-0526-8
关键词
Archaea; DNA replication; Genetics; PCNA; Structure; Thermococcus kodakarensis
资金
- National Science Foundation [MCB-0815646]
- National Institutes of Health [GM073336, GM53185, GH034559]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F32GM073336, R01GM053185] Funding Source: NIH RePORTER
Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. Most eukarya and euryarchaea have only one PCNA homolog but Thermococcus kodakarensis uniquely has two, designated PCNA1 and PCNA2, encoded by TK0535 and TK0582, respectively. Here, we establish that both PCNA1 and PCNA2 form homotrimers that stimulate DNA synthesis by archaeal DNA polymerases B and D and ATP hydrolysis by the replication factor C complex. In exponentially growing cells, PCNA1 is abundant and present at an similar to 100-fold higher concentration than PCNA2 monomers. Deletion of TK0582 (PCNA2) had no detectable effects on viability or growth whereas repeated attempts to construct a T. kodakarensis strain with TK0535 (PCNA1) deleted were unsuccessful. The implications of these observations for PCNA1 function and the origin of the two PCNA-encoding genes in T. kodakarensis are discussed.
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