期刊
EXTREMOPHILES
卷 13, 期 5, 页码 827-837出版社
SPRINGER JAPAN KK
DOI: 10.1007/s00792-009-0270-2
关键词
Deinococcus radiodurans; SMC; Nucleoid compaction; Resistance to ionizing radiation; Gyrase inhibitors; SbcCD complex
资金
- Agence Nationale de la Recherche [ANR-07-BLAN-0106]
- Centre National de la Recherche Scientifique
- University Paris-Sud 11
- Commissariat a l'Energie Atomique [CEA LRC42 V]
- Electricitede France
- Agence Nationale de la Recherche (ANR) [ANR-07-BLAN-0106] Funding Source: Agence Nationale de la Recherche (ANR)
Deinococcus radiodurans contains a highly condensed nucleoid that remains to be unaltered following the exposure to high doses of gamma-irradiation. Proteins belonging to the structural maintenance of chromosome protein (SMC) family are present in all organisms and were shown to be involved in chromosome condensation, pairing, and/or segregation. Here, we have inactivated the smc gene in the radioresistant bacterium D. radiodurans, and, unexpectedly, found that smc null mutants showed no discernible phenotype except an increased sensitivity to gyrase inhibitors suggesting a role of SMC in DNA folding. A defect in the SMC-like SbcC protein exacerbated the sensitivity to gyrase inhibitors of cells devoid of SMC. We also showed that the D. radiodurans SMC protein forms discrete foci at the periphery of the nucleoid suggesting that SMC could locally condense DNA. The phenotype of smc null mutant leads us to speculate that other, not yet identified, proteins drive the compact organization of the D. radiodurans nucleoid.
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