期刊
ACS NANO
卷 9, 期 10, 页码 9994-10004出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b03525
关键词
iridium; nanocrystal; peroxidase mimic; catalytic efficiency; immunoassay
类别
资金
- Michigan Technological University (MTU)
- Center for Nanophase Materials Sciences
Peroxidase mimics with dimensions on the nanoscale have received great interest as emerging artificial enzymes for biomedicine and environmental protection. While a variety of peroxidase mimics have been actively developed recently, limited progress has been made toward improving their catalytic efficiency. In this study, we report a type of highly efficient peroxidase mimic that was engineered by depositing Ir atoms as ultrathin skins (a few atomic layers) on Pd nanocubes (i.e., Pd-Ir cubes). The Pd-Ir cubes exhibited significantly enhanced efficiency, with catalytic constants more than 20- and 400-fold higher than those of the initial Pd cubes and horseradish peroxidase (HRP), respectively. As a proof-of-concept demonstration, the Pd-Ir cubes were applied to the colorimetric enzyme-linked immunosorbent assay (ELISA) of human prostate surface antigen (PSA) with a detection limit of 0.67 pg/mL, which is similar to 110-fold lower than that of the conventional HRP-based ELISA using the same set of antibodies and the same procedure.
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