期刊
EXPERT REVIEW OF PROTEOMICS
卷 11, 期 6, 页码 771-788出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.2014.947966
关键词
biomarkers; cardiovascular diseases; endogenous proteins; mass spectrometry; multiple reaction monitoring; post-translational modifications; protein isoforms; selected reaction monitoring; targeted proteomics
Selected reaction monitoring, also known as multiple reaction monitoring, is a powerful targeted mass spectrometry approach for a confident quantitation of proteins/peptides in complex biological samples. In recent years, its optimization and application have become pivotal and of great interest in clinical research to derive useful outcomes for patient care. Thus, selected reaction monitoring/multiple reaction monitoring is now used as a highly sensitive and selective method for the evaluation of protein abundances and biomarker verification with potential applications in medical screening. This review describes technical aspects for the development of a robust multiplex assay and discussing its recent applications in cardiovascular proteomics: verification of promising disease candidates to select only the highest quality peptides/proteins for a preclinical validation, as well as quantitation of protein isoforms and post-translational modifications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据