期刊
EXPERT REVIEW OF PROTEOMICS
卷 9, 期 2, 页码 125-128出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.12.8
关键词
2D separations; peptide retention prediction; reversed-phase HPLC; shotgun bottom-up proteomics
资金
- Natural Sciences and Engineering Research Council of Canada
Recent developments in bottom-up proteomics have supplanted the use of gel-based approaches in favor of multidimensional chromatographic separations of peptide mixtures followed by mass spectrometry analysis. This trend is driven by the desire to eliminate labor-intensive in-gel digestion procedures and increase proteome coverage through better recovery of proteolytic fragments. Introduction of reversed-phase reversed-phase 2D separation techniques is one of the major improvements that have made this possible. In this article, we review recent developments in 2D HPLC and highlight variations in reversed-phase HPLC separation selectivity that allow for superior peak capacity in peptide fractionation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据