期刊
EXPERT REVIEW OF PROTEOMICS
卷 6, 期 6, 页码 605-618出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.09.84
关键词
cancer; cell signaling; kinase; phosphatase; phosphoproteomics; protein phosphorylation; protein-protein interaction; quantitative mass spectrometry; stoichiometry of phosphorylation
资金
- NIH [RO1 HL 67569, PO1 HL 70694]
Phosphorylation of proteins is a predominant, reversible post-translational modification. It is central to a wide variety of physiological responses and signaling mechanisms. Recent advances have allowed the global scope of phosphorylation to be addressed by mass spectrometry using phosphoproteomic approaches. In this perspective, we discuss four aspects of phosphoproteomics: the insights and implications from recently published phosphoproteomic studies and the applications and limitations of current phosphoproteomic strategies. Since approximately 50,000 known phosphorylation sites do not yet have any ascribed function, we present our perspectives on a major function of protein phosphorylation that may be of predictive value in hypothesis-based investigations. Finally, we discuss strategies to measure the stoichiometry of phosphorylation in a proteome-wide manner that is not provided by current phosphoproteomic approaches.
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