期刊
EXPERT REVIEW OF PROTEOMICS
卷 6, 期 4, 页码 433-447出版社
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.09.55
关键词
biomarker; endogenous; mass spectrometry; multidimensional separation; peptide; peptidomics; proteomics; selective enrichment
资金
- National Natural Science Foundation of China [20675081, 20735004]
- China State Key Basic Research Program Grant [2005CB522701, 2007CB914104]
- China High Technology Research Program [2006AA02A309, 2008ZX10002-017]
- CAS [KJCX2.YW.HO9, KSCX2.YW.079]
- DICP
The peptidome, which is the low-molecular-weight subset of the proteome, has attracted increasing attention in recent years. However, with the interference of high-abundance protein components in complex biological mixtures (e.g., serum), selective extraction of endogenous peptides is the first and most important step before analyzing the peptidome. A number of methods and technologies have now been developed for the selective enrichment, fractionation, quantitative analysis of the endogenous peptides and their application in the potential biomarker discovery. This review will cover the methods and technologies developed in recent years for the peptidome analysis on the selective extraction, multidimensional separation and quantitative analysis, as well as their application for clinical diagnosis and biomarker discovery. The future prospects of the peptidome are also discussed.
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