期刊
EXPERT REVIEW OF PROTEOMICS
卷 5, 期 5, 页码 721-730出版社
EXPERT REVIEWS
DOI: 10.1586/14789450.5.5.721
关键词
activity-based probe; protease; proteolysis; proteomics
资金
- NCRR NIH HHS [U54 RR020843-057573, U54 RR020843] Funding Source: Medline
- NIBIB NIH HHS [R01 EB005011-04, R01 EB005011] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [U54RR020843] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB005011] Funding Source: NIH RePORTER
Traditional proteomics methodology allows global analysis of protein abundance but does not provide information on the regulation of protein activity. Proteases, in particular, are known for their multilayered post-translational activity regulation that can lead to a significant difference between protease abundance levels and their enzyme activity. To address these issues, the field of activity-based proteomics has been established in order to characterize protein activity and monitor the functional regulation of enzymes in complex proteomes. In this review, we present structural features of activity-based probes for proteases and discuss their applications in proteomic profiling of various catalytic classes of proteases.
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