期刊
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
卷 11, 期 7, 页码 735-740出版社
EXPERT REVIEWS
DOI: 10.1586/ERM.11.60
关键词
gene-expression profiling; RT-qPCR; single-cell biology; single-cell diagnostics; single-cell gene-expression profiling
类别
资金
- Assar Gabrielssons Research Foundation
- Goljes Memory
- LUA/ALF Vastra Gotaland
- Johan Jansson Foundation for Cancer Research
- Swedish Cancer Society
- Swedish Children's Cancer Society
- Swedish Society for Medical Research
- Wilhelm and Martina Lundgren Foundation for Scientific Research
- granting agency of the Czech Republic [GACR P303/10/1338, GACR 301/09/1752]
Gene-expression profiling has been successfully applied in various diagnostic applications, but its full capacity is yet to be realized. Samples are generally prepared from a mixture of different cells that are present in unknown proportions. Cells are, in many aspects, unique in their characteristics and this heterogeneity confounds the expression profile. The development of new and robust techniques to measure gene expression in single cells opens new avenues in molecular medicine. Today, gene-expression profiles of individual cells can be measured with high precision and accuracy, identifying different cell types as well as revealing heterogeneity among cells of the same kind. Here, we review practical aspects of single-cell gene-expression profiling using reverse transcription quantitative real-time PCR and its potential use in diagnostics.
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