期刊
ACS NANO
卷 9, 期 9, 页码 9217-9227出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b03876
关键词
drug delivery; vagina; paclitaxel; PLGA; mucosal surface
类别
资金
- NIH [P30-EY001765, R33AI07974, R01HD062844, GM056550]
- NSF [MCB-1157506]
- Raymond Kwok Family Research Fund, USA
- Johns Hopkins University Center for AIDS Research [P30AI094189]
- W.W. Smith Charitable Trust [A1302]
- China Scholarship Council
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1157506] Funding Source: National Science Foundation
Achieving sustained drug delivery to mucosal surfaces is a major challenge due to the presence of the protective mucus layer that serves to trap and rapidly remove foreign particulates. Nanoparticles engineered to rapidly penetrate mucosal barriers (mucus-penetrating particles, MPP) have shown promise for improving drug distribution, retention and efficacy at mucosal surfaces. MPP are densely coated with polyethylene glycol (PEG), which shields the nanoparticle core from adhesive interactions with mucus. However, the PEG density required to impart the stealth properties to nanoparticles in mucus, and thus, uniform distribution in vivo, is still unknown. We prepared biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles with a range of PEG surface densities by blending various ratios of a diblock copolymer of PLGA and 5 kDa poly(ethylene glycol) (PLGA-PEG(5k)) with PLGA. We then evaluated the impact of PEG surface density, measured using an H-1 NMR method, on mucin binding in vitro, nanoparticle transport in freshly obtained human cervicovaginal mucus (CVM) ex vivo, and nanoparticle distribution in the mouse cervicovaginal tract in vivo. We found that at least 5% PEG was required to effectively shield the nanoparticle core from interacting with mucus components in vitro and ex vivo, thus leading to enhanced nanoparticle distribution throughout the mouse vagina in vivo. We then demonstrated that biodegradable MPP could be formulated from blends of PLGA and PLGA PEG polymers of various molecular weights, and that these MPP provide tunable drug loading and drug release rates and durations. Overall, we describe a methodology for rationally designing biodegradable, drug-loaded MPP for more uniform delivery to the vagina.
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