期刊
EXPERIMENTAL PHYSIOLOGY
卷 97, 期 4, 页码 544-555出版社
WILEY
DOI: 10.1113/expphysiol.2011.062232
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资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0007540]
- National Research Foundation of Korea [2010-0007540] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
It has been suggested that mechanosensitive ion channels initiate myogenic responses in vessels; however, the molecular identity of the mechanosensitive ion channel complex is unknown. Although previous reports have suggested that epithelial Na+ channel (ENaC) proteins are mechanotransducers in arteries, experimental evidence demonstrating that ENaC proteins are mechanotransducers are not fully elucidated. The goal of the present study was to determine whether the ENaC is a mechanotransducer for the myogenic response by providing supporting evidence in the rat posterior cerebral artery (PCA). We measured the effect of ENaC inhibition on the pressure-induced myogenic response, Ca2+ concentration and 20 kDa myosin light chain (MLC20) phosphorylation. We detected expression of beta ENaC and ?ENaC subunits in rat PCA by Western blots and immunofluorescence. Inhibition of ENaCs with amiloride, ethyl isopropyl amiloride or benzamil blocked the myogenic response. Moreover, the myogenic response was inhibited in rat PCA transfected with beta ENaC and ?ENaC small interfering RNA. The myogenic response was inhibited by elimination of external Na+, which was replaced with N-methyl-d-glucamine. Amiloride and nifedipine inhibited the pressure-induced increase in Ca2+ concentration. Finally, MLC20 increased when the intraluminal pressure was raised, and the pressure-induced increase in MLC20 phosphorylation was inhibited by pretreatment with amiloride, and in arteries transfected with beta ENaC or ?ENaC small interfering RNA. Our results suggest that ENaCs may play an important role as mechanosensitive ion channels initiating pressure-induced myogenic responses in rat PCA.
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