期刊
EXPERIMENTAL PHYSIOLOGY
卷 93, 期 12, 页码 1233-1238出版社
WILEY
DOI: 10.1113/expphysiol.2008.043497
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- British Heart Foundation [PG/05/042, FS/06/027]
We examined the modulation of the cardiac L-type Ca2+ channel (LTCC) by the regulatory protein sorcin and tested the hypothesis that modulation occurred by direct interaction. Whole-cell patch-clamp recordings were made on native rabbit ventricular myocytes and HEK 293 cells expressing cardiac alpha(1C) subunits. In ventricular cells, sorcin increased peak current when using either Ca2+ or Ba2+ as charge carriers. In HEK 293 cells, sorcin increased peak current density when using Ba2+ as a charge carrier but not when using Ca2+. In ventricular myocytes, current inactivation (tau(fast), in ms) was slowed by sorcin with Ca2+ as the charge carrier, whilst in the presence of Ba2+ it was enhanced. In HEK 293 cells, sorcin significantly enhanced tau(fast), but no significant change was observed with Ba2+. This trend was mimicked by the truncated peptide, sorcin Ca2+-binding domain, which lacks the N-terminal domain. These data suggest that sorcin interacts with LTCC via its C-terminal domain, which alters current magnitude and tau(fast). These effects appear to be influenced by the prevailing experimental conditions.
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