期刊
EXPERIMENTAL PARASITOLOGY
卷 141, 期 -, 页码 93-97出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2014.03.004
关键词
Babesia microti; Beta-tubulin; Molecular phylogeny; Australia; Babesiosis; 18S
类别
In 2012, the first autochthonous Australian case of human babesiosis was reported, after microscopic examinations of blood samples revealed intra-erythrocytic parasites in a hospitalized 56 year-old man from NSW, who died in 2011 (Senanayake et al., 2012). Independent molecular analyses carried out in Australia and the USA, identified Babesia microti at the 18S ribosomal RNA (18S rRNA), and the beta-tubulin (beta-tubulin) gene loci. Here we present the details of a novel PCR-based assay for the beta-tubulin gene that was developed, during the original study, to corroborate the results obtained from the analysis of the 18S rDNA. The complete phylogenetic reconstruction, based on the two loci sequenced from the Australian clinical isolate, is also shown here for the first time. (C) 2014 Elsevier Inc. All rights reserved.
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