期刊
EXPERIMENTAL PARASITOLOGY
卷 131, 期 1, 页码 125-129出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2012.02.012
关键词
Theileria annulata; LAMP; Cattle; Diagnosis
类别
资金
- Key Project of Gansu Province [0801NKDA033, 1002NKDA035]
- NSFC [31072130, 30800820, 30972182, 31001061, 2010-S04]
- 973 Program [2010CB530206]
- NBCITS
- MOA [CARS-38]
- MOST, China
- State Key Laboratory of Veterinary Etiological Biology [SKLVEB2008ZZKT019]
- Specific Fund for Sino-Europe Cooperation
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria annulata, an economically important cattle disease in China that occurs in subtropical and tropical areas. These assays target the ribosomal RNA (18S rRNA) and ITS LAMP sequences. The primer set for each gene target consists of four primers, and each set recognizes six distinct regions on the target gene to allow for the highly specific detection of T. annulata. The specific ladder bands were amplified from the autologous genomic DNA of four Chinese-laboratory-preserved standard T. annulata stocks, and there were no cross-reactions with the genomic DNA of normal bovine blood and other protozoan species. The LAMP assays were sufficiently sensitive to detect 0.1 pg/mu l of genomic DNA. Furthermore, DNA extracted from blood collected from cattle experimentally infected with T. annulata (18-105 days post-infection) was amplified, demonstrating the high sensitivity of these primers. Of the 351 field samples collected from China, 24.5% were positively detected by two LAMP primers, and 18.2% were found to be positive for T. annulata infection by PCR. These results indicate that the LAMP assay could be a potential diagnostic tool for epidemiological studies of T. annulata infection in China. (C) 2012 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据