期刊
EXPERIMENTAL PARASITOLOGY
卷 127, 期 2, 页码 409-417出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.09.007
关键词
Toxoplama gondii; Edible shellfish; Nested-PCR; FLAG real-time PCR; Italy
类别
资金
- Ministry of the University and Research, Italy
- Regione Puglia, Italy [PE_100 (CIP PE_087) (2006-2008)]
- Bambino Gesu Hospital [RC 200702P002153]
- Ministry of Health, Italy
To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy). Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish. (C) 2010 Elsevier Inc. All rights reserved.
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