期刊
EXPERIMENTAL PARASITOLOGY
卷 127, 期 4, 页码 732-739出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2011.01.011
关键词
Giardia lamblia; Essential oils; In vitro activity; Drug action; Infectious diseases (ID); Ultrastructure; Protozoa; Drug development; Medicinal plants
类别
资金
- Fundacao para a Ciencia e Tecnologia (FCT)
The present work evaluates the anti-Giardia activity of Syzygium aromaticum and its major compound eugenol. The effects were evaluated on parasite growth, adherence, viability and ultrastructure. S. aromaticum essential oil (IC50 = 134 mu g/ml) and eugenol (IC50 = 101 mu g/ml) inhibited the growth of G. lamblia. The essential oil inhibited trophozoites adherence since the first hour of incubation and was able to kill almost 50% of the parasites population in a time dependent manner. The eugenol inhibited G. lamblia trophozoites adherence since the third hour and not induce cell lyses. The main morphological alterations were modifications on the cell shape, presence of precipitates in the cytoplasm, autophagic vesicles, internalization of flagella and ventral disc, membrane blebs, and intracellular and nuclear clearing. Taken together, our findings lead us to propose that eugenol was responsible for the anti-giardial activity of the S. aromaticum essential oil and both have potential for use as therapeutic agents against giardiasis. (C) 2011 Elsevier Inc. All rights reserved.
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