期刊
EXPERIMENTAL PARASITOLOGY
卷 127, 期 1, 页码 294-299出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.06.025
关键词
Albendazole sulfoxide; Anti-helminthics; CMFDA; GSH; GSSG; Praziquantel; Taenia crassiceps; Vital fluorescent staining
类别
资金
- DGAPA-UNAM [IN201003, IN216107]
- Consejo Nacional de Ciencia y Tecnologia (CONACYT), Mexico [V43629-M]
Using a murine model of cysticercosis caused by the Taenia crassiceps ORF strain, we developed a fluorescent quantitative evaluation of the action of two well known anti-helminthic drugs: albendazole sulfoxide and praziquantel. The fluorescence emitted by a biotransformed CellTracker Probe known as CellTracker Green CMFDA in the vesicular fluids of cysticerci was estimated, and the results were compared with macroscopic observations of the parasites. The pharmacological EC50 value of each drug and changes in the level of biotransformation of the fluorescent tracker caused by the drugs could be easily calculated. These drug-induced changes in biotransformation could be related to changes in the GSH/GSSG ratio of parasites. Both the cysticercosis murine model and the CMFDA biotransformation assay could be used as an in vitro screening method to evaluate potential or well known cysticidal drugs. (C) 2010 Elsevier Inc. All rights reserved.
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