期刊
EXPERIMENTAL PARASITOLOGY
卷 126, 期 4, 页码 476-481出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.05.024
关键词
Theileria sergenti; Theileria sinensis; MPSP gene; PCR; Detection; Differentiation
类别
资金
- National Natural Sciences Foundation of China [30800820, 30972182]
- 863 Project [2006AA10A207]
- National Natural Resource Platform Project [2005DKA21100]
- Sino-Europe Cooperation, MOST, China
- Key project of Gansu Province, Lanzhou, Gansu, China [0801NKDA033]
- State Key Laboratory of Veterinary Etiological Biology Project [SKLVEB2008ZZKT019]
- National Public Interests Research Institute [BRF080303]
Theileria sergenti and Theileria sinensis are closely related members of benign Theileria species found in cattle and yaks in China. They are morphologically indistinguishable. A polymerase chain reaction (PCR) targeting major piroplasm surface protein of T. sergenti and T. sinensis was developed in this study. The newly developed oligonucleotide primer set was able to specifically amplify the DNA of T. sinensis and in conjunction with primers for T. sergenti and these two species could be detected and distinguished. Specificity testing also revealed that there was no cross-reaction with the other tick-borne diseases Theileria annulata, Babesia ovata, Ana plasma marginate as well as bovine white blood cells. Phylogenetic analysis based on the MPSP gene sequences confirmed the specificity of PCR assays. The sensitivity of the methods was 0.1 pg DNA for the T. sergenti PCR and 1 pg DNA for T. sinensis PCR. Two hundred and thirty-six field blood samples from of cattle and yaks were collected from five different geographical regions in China where benign Theileria species have been found. T. sergenti was found in all five provinces but was absent from one county in Gansu Province. T. sinensis was only found in Gansu Province. In both counties in Gansu where the parasites co-existed, mixed infections were detected. Our results indicate that the PCR methods developed in this study are suitable for the detection and differentiation of T. sergenti and T. sinensis. (C) 2010 Elsevier Inc. All rights reserved.
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