期刊
EXPERIMENTAL PARASITOLOGY
卷 126, 期 4, 页码 592-602出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.06.031
关键词
Trematode; Schistosoma mansoni infection; Susceptible snails; Resistant snails; Molecular mimicry; Glycoprotein; Carbohydrate epitope; Oligosaccharide mapping; Mass spectrometry
类别
资金
- Deutsche Forschungsgemeinschaft [SFB 535, A15]
- Osaka University (Japan)
Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (beta 1-2)-linked xylose or terminal Fuc(alpha 1-3)GalNAc(beta 1-4)[+/- Fuc(alpha 1 -3)]GlcNAc(beta 1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host. (C) 2010 Elsevier Inc. All rights reserved.
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