期刊
EXPERIMENTAL PARASITOLOGY
卷 124, 期 3, 页码 350-356出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2009.11.004
关键词
Photolyase; Cryptochrome; Trypanosoma cruzi; Kinetoplast; Kinetoplastids
类别
资金
- Nacional de Ciencia, Tecnologia e Investigacion (Fonacit) [2007000960]
A photolyase-like protein gene found in the Tryponosoma cruzi genome database was cloned and expressed in Escherichia coli resulting in the formation of inclusion bodies. Antibodies against this protein were used to determine expression of the protein in the different forms of the parasite. It was visualized in the epimastigote form but not in amastigote or trypomastigote forms obtained from culture in Vero cells. In epimastigotes, this protein is located at the level of the mitochondrion associated to both sides of the kinetoplast. Sequence analyses indicated that this protein, as well as other photolyases from Leishmania spp. and Trypanosoma brucei are related to single-stranded photolyases or cryptochromes DASH. (C) 2009 Elsevier Inc. All rights reserved.
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