期刊
EXPERIMENTAL PARASITOLOGY
卷 122, 期 2, 页码 134-139出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2009.02.012
关键词
L. (V.) panamensis; L. (L.) amazonensis; L. (L.) major; L. (L.) donovani; Viannia subgenus; Leishmania subgenus; Leishmaniasis; Antileishmanial drug screening; GFP, green fluorescent protein; HIFBS, heat-inactivated fetal bovine serum; PMA, phorbol myristate acetate; PE, phycoerythrin; nagt, N-acetylglucosamine-1-phosphate; transferase gene
类别
资金
- Colombian funding agency, Colciencias [110, 257, 11150412982, 11150416514]
- USA NIH [AI-20486]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI020486] Funding Source: NIH RePORTER
Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein. The transfectants remained infective to macrophages, providing an in vitro model for screening antileishmanial drugs. This was demonstrated by flow cytometry of macrophage-associated GFP after exposure of infected cultures to known antileishmanial drugs, i.e. amphotericin B and glucantime (R). Fluorescence of GFP diminished progressively from infected cells with increasing drug concentrations used in both cases. The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus. (c) 2009 Elsevier Inc. All rights reserved.
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