期刊
EXPERIMENTAL PARASITOLOGY
卷 120, 期 1, 页码 118-122出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2008.06.002
关键词
vacuum infiltration; plant transient expression; PVX amplicon; secretory pathway; Toxoplasma gondii; Gra4
类别
资金
- ANPCyT [25286]
- CONICET [6336]
A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4(163-345)) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4(163-345) was placed under control of an additional PVX coat protein subgenomic promoter. in the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4163-345 accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants. (C) 2008 Elsevier Inc. All rights reserved.
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