期刊
EXPERIMENTAL NEUROLOGY
卷 225, 期 1, 页码 140-153出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.expneurol.2010.06.005
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Varicosities (VRs) are ubiquitous neuronal structures that are considered to serve as presynaptic structures. The mechanisms of their assembly are unknown. Using cultured Aplysia neurons, we found that in the absence of postsynaptic targets, VRs form at the leading edge of extending neurites when anterogradely transported organelles accumulate within the palm of the growth cone (GC) at a rate that exceeds their utilization by the GC machinery. The aggregation of excess organelles at the palm of the GC leads to slowdown of the GC's advance. As the size of the organelle clusters increases, the rate of organelle sequestration diminishes and the supply of building blocks to the GC resumes. The GCs' advance is reinitiated, leaving behind an organelle-loaded nascent VR. These mechanisms account for the recurrent deposition of almost equally spaced VRs by advancing GCs. Consistent with the view that VRs serve as ready-to-go presynaptic terminals, we found that a short train of action potentials leads to exocytosis of labeled vesicles within the varicosities. We propose that the formation and spacing of VRs by advancing GCs is the default outcome of the balance between the rate of supply of growth-supporting resources and the usage of these resources by the GC's machinery at the leading edges of specific neurites. (C) 2010 Elsevier Inc. All rights reserved.
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